Enzyme technology (2020)
Samuel McEwen Walsh
Autumn (13-week period)
Language of instruction
Competences corresponding to participation in the course Biochemistry are recommended.
The objective of the course is to provide students with an understanding of enzymes, as they are produced and applied in biotechnological and chemical engineering industries.
- Classification of enzymes
- Enzyme kinetics
- The influence of pH, temperature, ion strength, inhibitors, etc. on the velocity of an enzyme-catalysed reaction
- Enzyme stability
- Enzyme production and purification
- Application of enzymes in solution
- Application of immobilized enzymes
The purpose of the laboratory element (corresponding to 1.5 ECTS) of the course is to entrench an understanding of enzyme catalysed process stages and reactions in an industrial context.
On completion of the course, the student is expected to be able to:
- Describe important technological applications of industrial enzymes
- Describe the principles behind application of solubilized or immobilized enzymes in batch reactions or reactors and assess the advantages and disadvantages of the two techniques
- Explain the impact of temperature and pH on enzymes' stability and reaction kinetics
- Apply Michaelis-Menten-based enzyme kinetics in the determination of reaction rates
- Explain enzyme purification strategies
- Classify an enzyme in compliance with international standards
- Calculate enzyme kinetic parameters, Vmax and Km, on the basis of experimental data
- Calculate and explain the influence of enzyme inhibitors on rate and yield
- Analyse and assess experimental enzyme kinetic data from scientific literature
Seminars and laboratory exercises
Qualifications for examination participation
- Participation in laboratory exercises and acceptance of associated mandatory papers.
All course work shall be executed and submitted pursuant to guidelines set out by the course manager.
Examination and aids
Written examination. Duration of examination: 4 hours.
Permitted aids: Textbook, notes, laboratory reports and mathematical program/spreadsheet. No access to the internet.
The form of examination at a 3rd attempt may vary from the above.
The 7-pont grading scale